Sterile technique may include the use of sterile equipment, sterile gowns, and gloves (Perry et al., 2014). In health care, sterile technique is always used when the integrity of the skin is accessed, impaired, or broken (e.g., burns or surgical incisions). It is also used when performing a sterile procedure at the bedside, such as inserting devices into sterile areas of the body or cavities (e.g., insertion of chest tube, central venous line, or indwelling urinary catheter). Sterile technique is most commonly practised in operating rooms, labour and delivery rooms, and special procedures or diagnostic areas. Principles of sterile technique help control and prevent infection, prevent the transmission of all microorganisms in a given area, and include all techniques that are practised to maintain sterility. In the literature, surgical asepsis and sterile technique are commonly used interchangeably, but they mean different things (Kennedy, 2013). Sterile technique is a set of specific practices and procedures performed to make equipment and areas free from all microorganisms and to maintain that sterility (BC Centre for Disease Control, 2010). Surgical asepsis is the absence of all microorganisms within any type of invasive procedure. Perform your experiments as rapidly as possible to minimize contamination.1.5 Surgical Asepsis and the Principles of Sterile Technique Surgical AsepsisĪsepsis refers to the absence of infectious material or infection.Be careful not to talk, sing, or whistle when you are performing sterile procedures.Use only sterile glassware and other equipment.If you remove a cap or cover, and have to put it down on the work surface, place the cap with opening facing down.Return the cover as soon as you are finished. until the instant you are ready to use it and never leave it open to the environment. Never uncover a sterile flask, bottle, petri dish, etc.Always cap the bottles and flasks after use and seal multi-well plates with tape or place them in resealable bags to prevent microorganisms and airborne contaminants from gaining entry.Do not unwrap sterile pipettes until they are to be used. Use sterile glass or disposable plastic pipettes and a pipettor to work with liquids, and use each pipette only once to avoid cross contamination.Avoid pouring media and reagents directly from bottles or flasks.Wipe the outside of the containers, flasks, plates, and dishes with 70% ethanol before placing them in the cell culture hood.Always wipe your hands and your work area with 70% ethanol.Leave the cell culture hood running at all times, turning it off only when they will not be used for extended periods of time. Using a Bunsen burner for flaming is not necessary nor is it recommended in a cell culture hood.You may use ultraviolet light to sterilize the air and exposed work surfaces in the cell culture hood between uses.For routine cleaning, wipe the work surface with 70% ethanol before and during work, especially after any spillage.Before and after use, the work surface should be disinfected thoroughly, and the surrounding areas and equipment should be cleaned routinely.The work surface should be uncluttered and contain only items required for a particular procedure it should not be used as a storage area.The cell culture hood should be properly set up and be located in an area that is restricted to cell culture that is free from drafts from doors, windows, and other equipment, and with no through traffic.The simplest and most economical way to reduce contamination from airborne particles and aerosols (e.g., dust, spores, shed skin, sneezing) is to use a cell culture hood.
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